Some of extracellular matrices (ECMs), such as heparan sulfate proteoglycans (HSPGs) are involved in extracellular dynamics of morphogens/secreted signaling proteins including Wnt.  However, HSPGs have been considered as static and uniform scaffolds for morphogens, mainly based on Drosophila genetics.  We have revealed that some of HSPGs form clusters that accumulate Wnt ligands (Mii et al., Nat. Commun. 2017).  Recently, we noticed that Wnt and HSPGs dynamically and mutually regulate their localization on cell surface, and this coupling between Wnt and HSPGs could be a molecular basis of morphogenesis of multicellular systems.  In this study, we will focus on molecular mechanisms underlying the dynamic change of localization of HSPGs and Wnt during an establishment process of planar cell polarity (PCP) in the neural plate of Xenopus embryos, through development of live-imaging probes for HSPGs and quantitative imaging.  We will also examine roles of clustering of HSPGs with in vitro experimental system.  Furthermore, coupling of Wnt and HSPGs in a physiological condition will be examined with zebrafish.  Overall, this study aims to revise our view that HSPGs as just a static and uniform scaffold.