ECM is responsible for multimodal information transmission, and it has been difficult to intrinsically understand its role by separating physical (e.g. viscoelasticity) and chemical (e.g. signaling molecules) factors. In this study, we explore the essence of the ECM using artificial ECM proteins and synthetic polymer hydrogels designed to enable the separation of modalities. In particular, the phenomena of nonlinear viscoelastisity (Strain Stiffening and Strain Softening) caused by the mechanical coupling between cells and ECM at the molecular-cell-tissue level will be understood in a hierarchical and integrated manner. Furthermore, extracellular information molecules and functional factors identified in Groups A01 and A03 will be incorporated into polysaccharide nanogels with molecular chaperone functions and structured with hydrogels to develop designer matrices with three-dimensional control of viscoelasticity and physiological activity. These designer matrices will be applied to models of organoid formation and organogenesis, and the contribution of each ECM modality to life phenomena will be clarified. Furthermore, ECM-inspired biomaterials will be created based on the scientific principles revealed through the research.