We have previously reported the means for cerebral, retinal, and limb bud organoids from pluripotent stem cells (ES/iPS cells). Organoid technology enables the recapitulation of organogenetic processes in vitro. However, the application of organoid technology is still limited to a few organs. The ECM is known to undergo dynamic mechanical and chemical changes during organogenesis, and designing an artificial ECM that can control these properties of the ECM in a spatiotemporal manner would allow reproducible induction of more biologically complex tissues than is possible with existing organoid technology.

In this study, we will collaborate with other research groups to visualize ECM dynamics during organoid formation. In addition, by designing artificial ECMs that can control cell adhesion, extracellular mechanical properties, biodegradability, and material permeability, we will develop new and unprecedented organoid technologies. In particular, we will focus on the induction of early embryo-like structures, brain organoids with cranial tissue, and intestinal muscle organoids that oscillate synchronously.