Multicellular morphogenesis requires precise spatiotemporal control of collective cell migration. Our research aims to elucidate the underlying mechanisms from the perspective of the extracellular matrix (ECM) as a dynamic “information field.” Focusing on previously unexplored sex-specific differences in ECM remodeling, we use Drosophila genitalia development as an in vivo model to investigate how ECM dynamics are linked to collective cell migration. By integrating live imaging, matrisome analysis, and molecular genetics, we seek to reveal how local ECM remodeling regulates the timing and synchronization of collective cell movement. This study will redefine the ECM as a multidimensional regulator of cell behavior and provide new conceptual insights into not only developmental morphogenesis but also pathological processes such as cancer invasion and fibrosis.